edta mechanism of action anticoagulant edta 3k additives in blood test tubes
[
P
roduct
N
a
me]
Tripotassium EDTA
[Formula] EDTAK3·2H2O
[
P
ack
age
Specification
]
P
lastic bottle, 500g/bottle
[Performance] White crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight of 442.56, pH value of 7.3±1 (5% aqueous solution, g/ml,), solubility in water ≥60% (g/ml, at 25ºC). Stable at room temperature. The main content of tri-potassium
EDTA ≥99.0%. See the product test report for other indicators.
EDTA
tripotassium powder
[
Application
]
This product is specially used for in vitro anticoagulation of blood, and is an anticoagulant additive for clinical blood test.
It is used for blood sample pretreatment (anticoagulation treatment) in the process of clinical blood collection and test.
[
Scope of application
]
EDTA salt can protect the cellular components of the blood, does not affect the white blood cell count and size, has the least effect on the red blood cell morphology, and can inhibit the aggregation of platelets, suitable for general hematological tests. Except for the isolation and examination of platelets, it is
not suitable for coagulation test and platelet kinetic energy test, nor for the determination of calcium, potassium, sodium, iron, alkaline phosphatase, creatine kinase and leucine amino peptidase and PCR test.
[Method of use] the product is dissolved with deionized water (distilled water), prepared into a solution with a certain concentration and content, and then added into the blood collection
tube.
[
D
osage] the recommended anticoagulant dosage range in the industry standard of vacuum blood collection is 1.5-2.2mg
t
ripotassium EDTA per ml of blood. The anticoagulant effect of micro blood collector is the same. The company recommends 1.8-2.0mg of Tripotassium
EDTA
to anticoagulate 1ml blood for one-time venous vacuum blood collection
tube
, and 18mg of anticoagulation 1ml blood for peripheral blood anticoagulation.
Example table:
tripotassium
EDTA solution preparation and common additives scale
|
Volume of blood collection tube(
ml
)
|
2
|
3
|
4
|
5
|
6
|
7
|
8
|
|
Adding
amount of
Tripotassium EDTA
(mg)
|
3.0-4.0
|
4.5-6.0
|
6.0--8.0
|
7.5--10.0
|
9.0--12.0
|
10.5-14.0
|
12.0-16.0
|
|
Volume of a
dditive solution
amount(ul)
|
20
|
30
|
40
|
50
|
60
|
70
|
80
|
|
Concentration
of t
ripotassium
EDTA(
g/l
)
|
150-200
|
150-200
|
150-200
|
150-200
|
150-200
|
150-200
|
150-200
|
Hubei New Desheng is a professional manufacturer of EDTA dipotassium and tripotassium professional manufacturers, has a complete quality control system and after-sales service guarantee, if have procurement needs, welcome to inquiry and purchase.